Part:BBa_K2016001:Design

Medium constitutive E. coli promoter with included RBS - ready for cloning

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 38
  

Design Notes

Strong promoter included at the beginning of the sequence, followed by a spacer and RBS site. Optimised for E. coli - ready for direct cloning.

Source

source